FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_251_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590925
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT3400.017.3009821
GTCGGTT2804.5602064E-916.0651971
TCGACCG3058.62201E-1015.83615831
CGACCGT3101.8202627E-814.46672332
CCGGTAT4002.6866473E-912.9757371
TCGACGC4104.0563464E-912.62175143
GTCGACT3305.86806E-712.5825331
GTCCGTT5257.2759576E-1212.5226151
GGCGTTT6000.012.1106871
GTCGCTT6400.011.8944251
CCCGAAT5258.731149E-1111.863531
GTCGAGA2409.0008375E-411.5339891
GTCCGTA5854.9112714E-1111.2382451
TCGCGTT2150.005308210411.2362962
GTCCGGG8250.010.9048621
GTCGTGA4157.8348785E-710.8391691
GTTCGGA5502.386514E-910.6951531
GTTCGAC4209.49487E-710.67863429
CCCGAGT5552.7703209E-910.59881
GTACGAT3602.1105669E-510.5728231