FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6723865
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT6900.011.02564749
GATTCCG8400.010.3115252
TCGATTC8150.010.1854850
CGCGAGT3450.00186293928.9797222
TTCTCGA9901.8189894E-128.70829547
ATTCCGT9253.45608E-118.61879353
CGGTACA8759.858923E-108.28976661
TCTCGAT9253.3105607E-108.22373848
TCACCCG13900.08.1721943
CGATTCC11103.6379788E-128.11556651
ATCTAAG13850.07.7047956
GGCGGTA9001.400258E-87.691265659
GTCTAAG10354.1654857E-107.67055844
ACCCGGC15350.07.6244765
GTTCGAT7352.2349486E-67.5411251
TTTCTCG11557.094059E-117.471322546
TCGAACC4150.0098417197.46479277
GAATCGA7051.0987329E-57.3239414
GTTTGAT38400.06.94645071
AATCGAG8151.1452901E-56.75774575