FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6723865
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG1400.0041367514.7840256
GTCCGCT7700.014.3795451
GTCGCAT4508.54925E-1113.0714051
GTCGAGA3801.8275387E-812.7476551
GTCCGGA8250.012.1626991
GTCGGTT6050.012.0101881
GTCGGAT7550.011.9155011
GTCGAAT5301.03682396E-1011.7512081
GTCGGAG13800.011.5335931
GTCCCGT11200.011.4306141
GTCCGTT8900.011.2744121
CGATTCG2450.001094752111.2675384
GTCGTTA5006.160917E-911.072251
GTCGTAT6402.1827873E-1110.8127431
GCCCGAT6909.094947E-1210.5306721
GTCCAGT32750.010.3538211
GTCGGGT8700.010.3404631
CTCGTAT9500.010.1981251
GTCGCTT8850.010.16521
GTCCTAT13050.09.8101841