FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6772601
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT7202.1464075E-109.60626749
TCGATTC8709.094947E-129.14482250
GGCGGTA9203.274181E-118.65257259
TCTCGAT9203.274181E-118.64538348
TAAATCG5008.7919856E-58.2612053
GATTCCG8454.2200554E-98.20323352
GACGTAA5351.8951054E-47.732685641
CGTCGTA5552.7224745E-47.49387651
CGGTACA11303.3833203E-107.336423461
CAATTCG5704.0041312E-47.24667173
TCACCCG13405.456968E-127.19259173
ATTCCGT9654.9001756E-87.18634853
CCCGTAT4850.0046279587.14361951
TAAGCGC4900.0054357847.026338620
GTCTAAG12353.237801E-106.987344
TTCTCGA9402.589677E-76.971009347
AAATCGG9503.217665E-76.88459164
TAATCGA7007.8845485E-56.8845914
GCGGTAC12807.1486284E-106.745798660
GTTAAAT31400.06.73071