FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6772601
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4450.018.60719331
CGACCGT5250.018.39949632
GTCGGTT5250.015.1570691
GTCCGTT8800.014.5467421
GTTCGAC5150.014.06830329
TTCGACC6150.012.90275230
GCCGTAT6500.012.7745191
GTCGAGA4551.4024408E-912.1662091
TCGACGC6400.011.85913843
CCCGAAT7400.011.6883981
TCTCGAC6951.8189894E-1210.920403541
GTCGGAT7550.010.5396831
CCGGTAT6251.6552804E-1010.5176881
GTCGATA3301.0203367E-410.48413851
GTCGAAA4804.6472633E-710.0909831
GTCCGGG14750.010.0860971
GTCGGAA9750.09.9357381
AGTTCGA7051.5097612E-109.78747228
GTCCAAT21600.09.7706351
GTCGCAA4251.223248E-59.768751