FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13515330
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA19350.09.8189561
ACTAGGC20050.09.09817711
GGCGGTA21450.08.875319559
CTCGATT16750.08.25837949
GATTCCG19150.07.78032552
TCGTTGA21100.07.718154452
GTCTAAG24750.07.670680544
CGCTAGT6059.563387E-57.413692533
GCGGTAC25700.07.12337460
CAACTAG30950.07.0065359
CTAGGCT26750.06.948099612
ACGGGCG28600.06.914236556
TCGATTC21300.06.82037350
ACTATCG17400.06.770609452
TATCGGA18450.06.764119654
TCTCGAT19600.06.704242748
CCGCTAG8351.5541511E-56.61956965
TCGACTG19850.06.58926347
GTTTATT85400.06.5717951
ACACACG34500.06.52818352