FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13515330
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6200.017.80667331
CGACCGT7200.015.33250332
GTCGGTT11350.013.717311
GTTCGAC9150.012.06572429
GTCGCTT19350.011.6221021
GTCCGTT16000.011.2443841
GTCGTTT26350.011.1606791
CCGGCTA13750.011.03993337
GTCGGAA23550.011.0185041
TCGACGC10550.010.79124443
GTCGGCT18950.010.771971
GTCCGAT18850.010.4620261
GGCGTTT20900.010.2635521
GTCGAAT11500.010.2290051
CTCGTAT16300.010.1883971
GTCGGCA28050.010.1142411
GTTCGGT17500.010.0828761
GTCGCGA3157.744782E-49.8851721
GTCGCAT10900.09.8398281
GTCCAGT62350.09.5997941