FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13398756
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT15700.010.57314449
ACTAGGC26000.09.79623611
CGATACG20400.09.449326529
TCGATTC17750.09.15962550
TCTCGAT17500.08.89220248
CGGTACA21000.08.88264461
GATTCCG17950.08.88017552
TACGATA23500.08.78821127
CCGCTTA17500.08.51855453
GTAGTCG24900.08.49913257
GTCTAAG24000.08.48520244
CTAGGCT28750.08.14089112
TCAACCG17550.08.0411643
TCACCCG25250.07.90640073
ATTCCGT18700.07.78651553
TAGATCG8403.7225618E-87.78550053
ACCCATG60050.07.73865276
TCGTTGA27350.07.72859752
AGTCGCC27350.07.720880559
GATACGG27350.07.677509330