FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13398756
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6950.020.3525131
CGACCGT8750.016.55913232
GTTCGAC9150.015.83606129
GTCGCGA2353.1304498E-614.7224411
TCGACGC11050.012.80061443
TTCGACC12050.012.31119930
CCCGTAT9900.011.88205051
CGATCGG9550.011.19853141
CCGGCTA20600.010.88608237
TCGCGTA6251.7462298E-1010.486966
GTCCGCT14950.010.4140341
GTCGTTT22800.010.3186231
CTCGACG14100.010.27636242
CGTGCGA9800.010.20933237
TCTCGAC13250.010.15433541
CGGGTAT10150.09.8850671
GTCCGAT24600.09.8448841
CGGATTA13100.09.74318164
GGCGTTT17850.09.6912421
AGTTCGA13900.09.679852528