FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9388101
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29300.050.864783
CGTATGC30250.050.8434071
ATGCCGT33400.043.489664
CCGTCTT32250.036.5008137
TGCCGTC41800.034.1731385
GCCGTCT38350.032.850556
GTATGCC47600.032.5388682
CGTCTTC47400.024.9800428
GTCTTCT64650.014.32177459
CGGTACA13850.08.98043661
GATTCCG12100.08.58938152
CTCGATT10500.08.56387249
GGCGGTA14300.08.2313959
TCGATTC12700.08.1713650
TACGCGA4250.00125117958.16264956
TTACGCG4300.0014095938.06474855
TCTTCTG113850.07.64892710
GCGTATA5907.1863025E-57.586530739
GCGGTAC16400.07.5834460
GTCTAAG17500.07.49637644