FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9388101
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGA10500.014.1670111
TCGACCG4650.014.0968831
GTCGGAT10900.012.3776211
GTCGGTT7850.011.457841
GTCCGTT10850.011.1593111
GTCGCTT12650.010.9387711
CGACCGT6101.1277734E-1010.74523832
GTCCAGT49650.010.590671
GTCCGGT14050.010.5874461
GTCGATT8450.010.2348721
GTCGCAT6858.185452E-1110.1003991
GTCGTAT9600.010.0898761
CCGGTAT9300.010.043381
CCCGGTT11900.09.8839621
GTCCGCT11200.09.5750881
GTCGTTT17050.09.5361391
GTCCCGT16350.09.5212471
GTTCGAC6554.3401087E-99.48098829
CTCGTTT25250.09.4533731
CCCGATT7758.731149E-119.373821