FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17376052
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT21850.09.18144249
TCTCGAT23550.08.37113748
CGGTACA23950.08.36557561
GATTCCG23500.08.11038352
TCGATTC23600.08.06274850
CGCGGTA9405.0567905E-108.05492817
TTCTCGA25150.07.54138347
ATTCCGT24650.07.454300453
GGCGGTA24600.07.036962559
GCGGTAC26550.07.0252860
TCACCCG34300.06.72310643
CCGGACA32300.06.7127412
CCCGGAC33750.06.62821211
TAGCGTA12201.3040335E-86.50496337
CGATTCC31800.06.429415751
GTCTAAG32050.06.354590444
GGAAAAT167300.06.3158921
GTTAAAT86900.06.25908231
GTTTTTT230100.06.2031381
GTTAGAT45750.06.1337311