FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17376052
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT15050.013.1043071
TCGACCG8500.012.988234531
CGACCGT8650.012.36362732
GTCCGCT22400.012.0482031
GTCGGAT19550.012.0347721
GTCCGAT18000.011.1488791
GTCGATT16400.010.5487711
GTTCGAC10450.010.23444929
GTCGTTT28500.010.19788551
CCCGTAT15350.010.1433121
GTCGAAT15400.010.1103811
GTCGTAT16550.010.0350361
AAGTTCG15400.09.6329527
GATCGCG6201.7897037E-89.45951340
GTCGGAA22400.09.4223131
GTTCGAT22800.09.4087631
GCCGTAT17450.09.319191
GTCGCAT13800.09.2768031
GTCGAGA11000.09.121811
GTCGCGA3804.2206628E-49.1052541