FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18453221
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT20550.010.43211349
TCTCGAT22750.09.4225548
TTCTCGA25900.08.7878647
GATTCCG24950.08.4693852
TCGATTC26500.07.961350
GTAGTCG17550.07.905336457
ATTCCGT27500.07.43520453
GTTTAAT83550.07.1317621
TCGACTG25150.07.1170457
CGCGGTA10757.603376E-97.04430217
CGGTACA27200.06.985308661
GTCTAAG30200.06.97205144
CGATTCC31700.06.775259551
TCACCCG36100.06.674783
GGCGGTA27500.06.67061959
ATCGACT29000.06.6473956
TACATCG35200.06.45427373
TTTCTCG37250.06.3011446
CCGGACA39350.06.210621412
GTTTATT116050.06.2091621