FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18453221
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG8800.018.03431331
CGACCGT11000.014.7404132
GTTCGAC12100.014.25637329
GTCGGTT14100.012.512921
GTCGCAA9400.012.1448951
CCCGTAT12800.011.3513361
TCGACGC15900.011.28282143
GTCGCAT13850.010.7405441
CGCGTAA7450.010.64983867
GTCCGTT24150.010.6003991
GTCGTAT19300.010.5755351
GTCCGAT24200.010.4355451
TCGCGTA7151.8189894E-1110.13172866
AAGTTCG18300.09.99176327
GTCGGAT26550.09.902771
AGTTCGA17900.09.8297328
GTCGCTT26300.09.7338271
GTCCGCT23500.09.5687051
GTCCAGT88000.09.5528121
GTCCGGA23950.09.53336051