FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834320
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT8750.011.46151349
ATTCCGT11300.09.81741553
GATTCCG10250.09.46644152
TCTCGAT10900.09.20028448
CGATACG8303.8198777E-119.12346429
TCGCGAA3804.4312712E-49.06087720
TCGATTC11000.08.80493350
CGGTACA12053.6379788E-127.74001161
TTCTCGA11851.6370905E-117.566197447
TACGATA11002.0190782E-107.509701327
GGCGGTA12152.7284841E-117.408420659
ATCGACT14050.07.3497276
ATTTGCG5250.00133418257.21206576
GATACGG11606.4574124E-107.121495730
CGATTCC13252.7284841E-117.06155751
CTCGAAT7957.194645E-66.97321221
GTCCGTT5500.0020357486.929631
TAAGCGC5500.00217505736.886266720
GTTTAAT38250.06.8843381
ACGGAAT11705.493348E-96.782237533