FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834320
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT9400.014.3555181
TCGACCG4408.6401997E-1012.54521331
CGACCGT4451.035005E-912.40362232
GTCGCTT10800.012.1742441
GTCGCAT6300.012.0827071
GTCGGAA12100.011.724131
GCCGTAA6950.011.4505191
GTCCGTT10450.011.2575721
GCCGTAT8300.011.2555781
GTCGGTT6800.011.1942741
GTCGGAT11400.010.9264681
GTTCGAC5703.9108272E-1010.894526529
GTCGATT10300.010.7496631
CCCGTAT5552.7739588E-910.5983461
GTCGGAC9550.010.5069531
GTCCAGT37700.010.3709661
CTATCGA4451.992723E-610.07967954
CGCTAGT2800.00325208049.8858521
TCGACGC5959.136784E-99.85657543
GCCCGTT14750.09.852341