FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5078432
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4802.5102054E-1012.25129449
TCGATTC6453.2492608E-89.11868650
GACGCTA3100.00738104858.94979255
GATTCCG6604.3645196E-78.40162852
TTCTCGA7051.439912E-78.31620347
TCTCGAT6553.6197325E-67.92125848
ATTCCGT7008.985904E-67.429016653
GTTTTAT41350.07.03911351
TTCGACG4900.00544830647.02459966
CGATTCA4900.00544986977.02439174
AATCGAA7552.8747641E-56.8382485
CGATTCC8251.240243E-56.721302551
GTTTAGT15151.4551915E-116.632841
CGGAAAT5750.00324573956.62886241
CGGTACA7451.699305E-46.491173761
GGATAAT14057.912604E-106.41226151
GTTAGAC7652.3006288E-46.34133671
GGCGGTA8208.3549035E-56.332046559
GGAAAAT47450.06.28024241
GTTTAAT24300.06.2742331