FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5078432
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT1202.395369E-623.0712221
TCGACCG3205.456968E-1217.25042731
CGACCGT3257.2759576E-1216.98386632
CCCGAAT5150.015.455481
GTTCGAC4151.8189894E-1214.96422529
GTCGGTT3801.2460077E-913.6605921
CCCGTAT4851.8189894E-1213.5573161
GTCGTTT8250.013.003781
TTCGACC4551.07320375E-1012.89042830
TCGCGTC2701.347057E-512.7832552
CGGCTAT3953.1286618E-812.2657131
AGTTCGA5207.8216544E-1111.94260228
TCGACGC4652.066372E-911.87067543
GTCCGTT6750.011.7919591
CTAATCG2050.003733357911.77892365
CCCGGAT10250.011.479341
TCTCGAC5452.1573214E-910.76088641
CGCCTAC3301.0174834E-410.4869191
TCCGCTA2700.00245538110.22660352
CTCGTTT13650.010.1411971