FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473086
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA5908.277493E-89.36556561
TTCGATA3450.00186281858.9796512
GGCGGTA6909.6229996E-88.52692459
ACGGGCG9001.5679689E-98.09561156
GCGGTAC7808.378447E-87.968752460
GTTTAAT21700.07.82352921
GTCTAAG7201.7289422E-67.6708644
ACGGATC4600.00294168437.4830432
CTTAGAT13651.8189894E-127.3609141
AATCGAG6151.2045608E-47.27627665
CGAAAAT5250.0012444477.2593841
GGGGAAT16900.07.1754271
AATTGAC12152.382876E-107.08279565
AATCGAA6855.9323233E-57.0352325
TCACCCG7552.8711387E-56.8388083
ATTCCGT6250.00105428246.6570353
AATCGTG5750.003478526.5851525
GTTCGAT5800.00355779966.57099441
AAATCGA8006.4153566E-56.45426944
GATTCCG6000.00505876636.35324852