FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473086
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT2452.8249815E-715.5364741
GTCGATT4052.2191671E-1013.6707041
GTCGGTT3608.558345E-913.45711
TCGACCG2651.1166758E-513.0182531
CCCGATT3508.5849024E-812.8529021
GTCGTAT4501.5428668E-811.5346571
CCCTAGT3954.1370185E-711.3886481
CGGCAAT3651.9802956E-611.3766461
TCGACGC3054.7165464E-511.31093843
CCCGTAT4351.1949487E-711.13690951
GTCGGAT5653.1832315E-1011.0242731
GTTCGAC2853.161031E-410.89421929
GTCGAAA2550.001493491910.8561471
CGACCGT2903.7137372E-410.70602932
GCCCGTT9450.010.6192061
GTCGTTA3955.1041316E-610.5125981
CGTCGCG2650.00210615610.4171623
GTCCCGT8050.010.3167111
GTCGGGA6402.5465852E-1010.2730531
CCGGTAT4401.6605154E-610.22391