FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14401929
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG15800.010.52677952
CTCGATT17150.09.88211749
TCGATTC19350.08.93971750
TCACCCG25800.08.5377263
CGGTACA21550.08.49710761
TCTCGAT17800.08.35474448
GGCGGTA20850.08.13674659
ATTCCGT20550.08.09751653
CGATTCC22900.07.565696251
GCGGTAC26650.07.25933760
GTCGAAT6754.4957473E-57.18526841
TTCTCGA21250.07.14032547
TCGACTG19400.06.9188327
GTTTAAT65250.06.63663961
TATTCGC10452.4015753E-76.5869387
TCACTAA42850.06.34536658
ACTAGGC17900.06.34465811
GTTTTTT173800.06.33863971
GTTTATT92450.06.29536771
GTCTAAG29450.06.212159644