FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14401929
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG6350.019.016131
CGACCGT8750.013.79939332
GTTCGAC9550.012.28294929
GTCGGTT11700.011.5343751
GTCCGCA19600.010.5927941
GTCCGTT18850.010.4635441
TCGACGC12250.010.42013143
GTCCGAT18450.010.3152951
CGATCGG8450.010.20661541
GCGATCG8800.010.19322940
CCCGAAT16700.010.1530121
GTCCAGT71300.09.9975381
GTCGGAT20850.09.9577341
GTCGTAT16350.09.9470761
GTCGTTT22600.09.7991151
GTCGGCT20900.09.4372171
GTCCGCT19000.09.2882071
GCCCTAT21650.09.2701211
TCTCGAC13300.09.07851741
TTCGACC13800.09.00016630