FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19189283
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT23900.010.99592149
TCTCGAT27000.09.860826548
CGGTACA31700.09.37173361
GATTCCG28300.09.30386852
TCGATTC29500.09.26263850
TTCTCGA30300.08.87768247
ATTCCGT30350.08.79357753
TCACCCG39300.08.5835213
GGCGGTA31350.08.17113259
GCGGTAC36150.07.644074460
CGATTCC35600.07.590706351
GTAGTCG17450.07.55440557
CGTGGGT41850.07.54324157
GTTTAAT91450.07.23569771
CCCGGAC41400.07.149985311
CCGGACA41150.07.109779412
TTTCTCG40500.07.074098646
GTCTAAG37150.06.875781544
TAAGCGC14102.0008883E-116.837825320
CCGCTTA14859.094947E-126.768697353