FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19189283
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG11350.019.45385631
CGACCGT12650.017.4536932
GTTCGAC13800.016.00009229
TCGACGC18750.012.14362643
GTCGGTT18050.012.0756061
TTCGACC18950.012.015930
CCGGCTA18000.011.30845737
TCTCGAC20650.011.02606641
TCGCGTA9400.011.00946766
GTCGTTT32800.010.653521
AGTTCGA21250.010.39064828
CGCGTAA9350.010.33050567
GTCGCAT15100.010.3105341
AAGTTCG21750.010.31026927
GTCCGCT23550.010.1368681
GTCCGAT24650.010.1055771
GTCCGGA24250.09.9869271
GTCCGTT22800.09.7115981
GTCGAAT18900.09.7019641
CGATCGG12550.09.62101741