FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16354197
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT18900.010.97707249
GATTCCG22350.010.23109552
TCTCGAT21750.09.53814148
GGCGGTA22500.09.383759
CGGTACA24700.08.95103861
TCGATTC24850.08.90769650
TTCTCGA24450.08.8861147
ATTCCGT26600.08.59994753
GCGGTAC25200.08.224442560
GTCTAAG29250.08.024344
CCCGGAC31500.07.75816711
ACGGGCG32050.07.468472556
CGTGGGT36300.07.358729457
CCGGACA31950.07.21801712
TCACCCG31200.07.17116933
CGATTCC30850.07.07525951
CGGCCCG41250.06.92621568
CGCGGTA7603.152495E-56.793469417
CGGGAAT22200.06.7101521
ACCCGGC36750.06.6503865