FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16354197
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT19050.013.0761851
GTCCGCT21950.012.2942961
CGCGTAT3501.1979064E-611.8619681
CGATCGG11300.011.6011641
GTCCGGT22000.011.1655271
TCGACCG7850.010.98738831
GTCGGAT19250.010.7836081
CGCGTAA6503.274181E-1110.61424267
CCCGAAT18250.010.4266171
TCGCGTA6951.0913936E-1110.42330766
GCGATCG13150.010.23183940
CGACCGT9450.010.22177832
GTCGGTT14050.010.0960411
GTCGCTT21700.010.044411
GTCGGCT25950.09.7326041
GTCCGTA20100.09.6390791
GTCGTAT16550.09.6161991
GTCCGGA20700.09.5268231
GTCGTTT28700.09.5233291
CGTGCGA13150.09.44483337