FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13237564
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGTA18100.09.18146259
CGATAAT6402.750403E-78.6604771
CTCGATT15500.08.47928149
GATTCCG18800.08.47885552
CGGTACA22100.07.97273261
GCGGTAC21000.07.731580760
GTCTAAG23700.07.719041344
ACGGGCG25900.07.368099756
ATTCCGT19900.06.968416753
TTCTCGA18850.06.951952547
GTTTAAT66300.06.8970311
TCGATTC20700.06.68498850
TCTCGAT18200.06.650904748
GTTTATT89000.06.5780691
TAGCATA34100.06.45973069
TCACCCG24600.06.43588453
CGCGGTA7056.4170937E-46.346268717
ACACACG32150.06.251499752
GCGCTAG6650.00224674126.22560632
CGATTCC24400.06.106876451