FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13237564
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT11950.016.2158411
GTCGCAT9800.013.4176641
TCGACCG7600.013.16469131
CGACCGT8450.013.06451432
GTCGGAT14050.013.0532571
GTCCGCT16350.011.8519451
GTCGTTT24100.011.0558731
GTCGAAT11150.010.55172351
GTCCGTT16000.010.3810341
GTTCGAT17250.010.2305841
GGCCTAT15000.010.1503441
GTTCGAC10000.010.00516429
GTCGTAT13850.09.9937751
GATCGCG5603.7603968E-89.85726840
TCGCGAG6001.0477379E-89.77471842
CCCGAAT15300.09.7251511
CTCGTAT20250.09.5693481
GTCGGGA19000.09.4704171
GTCCGAA12450.09.4499371
CCGGAAT16150.09.4275641