FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950807
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG6251.8189894E-1211.64323352
CTCGATT6251.6734703E-1010.51404149
CGGTACA7453.6379788E-1210.20017161
GTTGCGC3552.2435669E-49.69697329
CCGCTTA4057.535599E-59.41657353
GGCGGTA7504.5838533E-109.23071259
TTCTCGA7504.9294613E-109.195127547
GCGCTAG3050.0067472449.04823332
CCGGTAA3050.00687388949.02776213
TCTCGAT7302.7903297E-99.00129148
TCGAACC3855.062518E-48.9407327
ACTAGGC6554.527101E-88.93417810
CCGCTAG3100.00762679578.91411465
TCGATTC7851.0986696E-98.81315450
GCGGTAC9557.2759576E-128.6798860
CGCTAGT3200.0099918158.62479533
CGCTTAT4855.658769E-58.5785254
ATTCCGT7504.033427E-88.32082653
ACACACG12100.08.305140552
CCGATAA4300.00141846688.0594531