FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4950807
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2251.0913936E-1121.46662531
GTTCGAC2855.995389E-915.73681329
CGACCGT2705.2619725E-815.33268532
TCGACGC3654.3655746E-1115.12310643
CGATCGG2756.5905624E-815.05390841
GTCCGTT5750.013.8423381
GTCGGTT3851.5152182E-913.4827961
GTCCGAT5600.012.9771911
CGTACCG1950.00256020412.387473
GTCGGTA6750.012.3042991
CTCGTAT6750.011.791621
TCGGTTA3256.975728E-611.6826822
CGCCTAT3003.8603263E-511.5352811
CGTGCGA3003.9822156E-511.50021137
GCCGTTT9200.011.2845131
GTCCGGT7700.011.2356631
GATCGAC2802.684638E-411.0881445
GTCCGAG7500.011.073871
CTCGACG5057.532435E-910.93056242
GCGATCG3803.3290762E-610.89493740