FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10318631
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT12650.010.66279749
GATTCCG14500.09.55914152
CGCGGTA5503.157602E-79.38685717
CGATAGT4201.1305908E-49.0730591
TCTCGAT14650.08.97030248
AATAGCG9253.6379788E-128.95219335
CGCGAAC3100.0078587998.88175613
TCACCCG20300.08.6477183
CGGTACA17300.08.58692261
TTCTCGA16100.08.14026247
TCGTTGA14600.08.06961752
GGCGGTA17750.07.99551659
TCGATTC16100.07.95017350
CGGTAAT7453.598234E-77.85437519
ACTAGGC14250.07.72906111
ATTCCGT15750.07.70323853
CGATTCC17550.07.50300951
GTCTAAG19950.07.439500344
CTCACTA27850.07.4155077
GTTTATT62400.07.328241