FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_212_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_212_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4325944
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT5251.4955003E-79.88142349
GGCGGTA5704.674257E-89.71901659
TCGATTC5351.960907E-79.69931550
ATTCCGT5856.9100133E-78.89170153
TAATCGA4452.2728268E-48.507394
TTCTCGA6503.7274913E-78.48906447
ATAATCG4109.4843586E-48.3940143
TCTCGAT5806.0977127E-68.34790548
CCGGACA7757.9566234E-87.99317612
TCACCCG8759.213181E-97.86639073
CGATTCC7654.905978E-77.702731651
GTTCGAT4600.00276585837.53028351
GATTCCG6452.4444184E-57.52359852
GGGTAAT6653.694902E-57.2924851
GTTTATT26250.07.2577591
GTTTAAT22550.07.2197221
GGTAATC5250.00133620537.21085832
GTTTAGT12505.2750693E-117.2049751
CGGTACA6401.8802186E-47.017899561
GCGGTAC6956.844121E-56.959078360