FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_212_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_212_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4325944
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT2503.6777055E-715.17926632
TCGACCG2605.7273246E-714.59679731
GTCGCTT5600.014.210681
GTCGTTT7200.013.9360771
GTCGGTT3354.8479706E-813.4268341
GTCGCAA2608.902138E-613.307661
CCGGTAT3952.228262E-913.1392091
CGCCTGT5350.012.9345481
CCCGAAT5307.2759576E-1212.4037441
GTCGGCT7150.012.0978731
GCCCGAA5451.2732926E-1112.0623561
GTCGTAT4702.3337634E-911.7786951
CGGCTAT2953.2567248E-511.72878551
GTCCGAA4207.362178E-811.5333051
TTCGACC3308.308363E-611.50050730
GTCCGGT6401.8189894E-1211.3530981
GCGGTAT3451.31868255E-511.03185751
GCCTTAT9250.010.8475421
TCGGCTA2550.001526300810.8278872
GTTCGCT4804.0017767E-810.8124741