FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_211_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_211_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353541
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT5209.495125E-1011.30761149
GATTCCG5956.548362E-1111.066236552
TCGATTC6051.0459189E-910.29396850
TCTCGAT5958.751158E-99.88216948
TTCTCGA6101.4095713E-79.04667547
TCACCCG7707.91988E-98.492743
GTTAAAT20350.08.1718351
GTTTAAT20150.07.73713641
GTTCGAT5452.2170061E-47.6282961
ATTCCGT7452.708608E-67.44487953
CCGGACA8403.0726733E-77.375019612
CCCGGAC8951.1383963E-77.30635111
TTTCTCG7603.88057E-67.268090246
CGATTCC8251.6280483E-67.141070451
GACAATC12801.05501385E-106.9911452
GTTTTTT56500.06.92903471
GTTTGAT27150.06.89075371
CTTAGAT11654.603862E-96.839821
GTTTTAT32850.06.74974541
CGGAAAT5650.00270426536.7450781