FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328670_211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328670_211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353541
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2901.8189894E-1219.03408831
CGACCGT2901.8189894E-1219.03343232
GTTCGAC3501.8189894E-1216.75679429
GTCGAGA2807.9418896E-814.8264481
TTCGACC3901.2732926E-1014.15355130
GTCGGTT4354.7293724E-1113.5199021
TCGACGC4408.6401997E-1012.54490643
AAGTTCG4501.2314558E-912.26641127
AGTTCGA4501.2314558E-912.26641128
GTTCGAT5401.0913936E-1112.1723311
ATACCGC2000.003101674612.0743328
GTCGTTT7250.011.9293271
TCTCGAC5005.16593E-1011.72921841
GTCGCAT3003.8723978E-511.5316811
GTCGTTA3359.5290325E-611.3595671
GCCCTAT7350.011.2963411
GTCCGCT5552.3283064E-1011.2200151
CGATCGG2853.161559E-410.8940141
CCCGGTT4552.2300082E-710.64462951
TAGTTCG2600.00180107610.6149243