FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11315392
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT13250.09.63706349
TTCTCGA14400.09.58688547
TCTCGAT14750.09.35894448
GATTCCG14800.09.34002852
CGGTACA14950.09.03094161
TCGATTC15700.08.13409150
ATTCCGT16650.07.88713553
GTTTAAT52450.07.5360031
GCGGTAC19150.07.049700760
CGCTAGT5000.00644435556.904178633
ACTAGGC14001.6370905E-116.885323511
GGAAAAT102650.06.8567571
CGATTCC21150.06.69700951
CCGGACA23900.06.625633712
GGCGGTA16201.8189894E-126.62387259
ATAGATC22450.06.5946912
GTCGAAT5850.00386399476.5195641
TACATCG21200.06.4967083
TCACCCG23500.06.4469463
AAATCGA21050.06.38069634