FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11315392
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG5850.015.33165231
GTCGCTT15600.014.560831
CGACCGT6900.012.4986332
GTCCGAA10800.011.3250891
GTCCGAT14100.011.1529761
TCGACGC9000.011.11367943
GTTCGAC7850.010.98605629
CCCGTAT8600.010.97141551
GTCCGTT14150.010.3726611
GTCGAAA8450.010.3390511
GTCGGAT15550.010.3377211
GTCGTTT17550.010.1552461
GTCCAGT53300.010.0314011
GTCCGCT14750.09.71381
GCCCTAT19450.09.7022291
GTCGTGA10150.09.6402741
GTCGAGA9100.09.6005471
GTTCGGT17000.09.4559741
GTCCGGA17000.09.4559741
CCCTAGT8605.456968E-129.3460211