FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12529431
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT17250.09.803149
CGGTACA20200.09.08161761
TTCTCGA17850.08.89413647
TCTCGAT18400.08.44016848
GATTCCG18750.08.29359952
GGCGGTA23000.08.2764859
CGCTAGA8555.484253E-98.08916666
ATTCCGT19300.07.878203453
TCGATTC21750.07.6168150
TCACCCG24800.07.4976763
GTCTAAG24950.07.331297444
CCGGACA26350.07.055341212
CGATTCC23700.06.996576351
CCCGGAC29150.06.85044111
ACACACG31650.06.769394452
CCGCTAG9351.6784979E-66.6573465
GCGGTAC27650.06.634138660
GCGTATA8855.338021E-66.62042239
CATATGC33400.06.596876612
GTTTAAT58150.06.5582491