FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12529431
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG7150.020.26311531
CGACCGT8200.017.66844732
GTTCGAC9400.014.67895529
GTCGGTT10650.012.4665021
TTCGACC12000.012.36090330
TCGACGC11700.012.08644543
GTCCGGA18000.012.0345481
GTCCGAA12150.011.7901191
CGATCGG9150.011.30843441
TCGCGTA5804.3655746E-1111.29904666
GTCGGAT17700.011.2515461
AAGTTCG13450.011.027390527
GCGATCG9900.010.80016940
AGTTCGA13600.010.65201428
GTCCGTT14200.010.3340731
GTCGCAT10850.010.3045931
TCTCGAC14500.010.22828341
GTCGTAT13000.010.2129421
GTCCGCT15400.010.2094521
CCCGAAT15500.010.1435841