FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15825416
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA21150.08.34647661
GGCGGTA22150.08.28140659
CTCGATT17750.08.16578649
GATTCCG20600.07.54888552
GCGGTAC24550.07.189794560
TCGATTC21900.07.09164650
GTCTAAG25350.07.080016644
TCTCGAT20650.06.851897248
TCGCGAA5600.00262662076.76406420
CGCTAGT7703.6193185E-56.72644533
CGCCTAG7401.6265082E-46.51333329
ACGGGCG31750.06.324852556
GTTAAAT76850.06.3137391
GGGGAAT55300.06.0165641
ACACACG35750.05.993122652
GTTTGAT93250.05.8723331
TTCTCGA22350.05.86842947
ATTCCGT23100.05.834318653
CGGGAAT23800.05.82485961
GGAAAAT152550.05.7933591