FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15825416
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT13850.014.874421
TCGACCG7700.012.54427431
GTCGCTT21950.011.7715861
GTCCGGT23650.011.2207081
GTCGGAT18050.010.8329961
GTTCGAC10100.010.58811229
GTCCGAT15800.010.38672351
CGACCGT9000.010.34902732
CGCGTAT4056.1811315E-610.34579851
GTCCGTT19350.010.2856491
CCGCGAA3803.4676385E-510.10757351
GTCGATT14650.010.0103551
GTCCGTA17600.09.721231
GTCGCAT12650.09.660851
GTTCCGT18050.09.4788711
GGCGTAT15600.09.400751
GTCCGGA20100.09.2069881
GTCGTTT26250.09.1782021
CGGGTAT14950.09.1088011
GTCCAGT79100.09.0493041