FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4171235
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG2850.00378538839.70580565
GAATCGA4704.092408E-69.5270184
CGCTAGA3000.0057780549.22051466
ATTCGAA3400.0016226339.1168295
CTCGATT4351.7297416E-48.72458249
GTCGGTC3600.00257385458.6655121
TGAATCG4754.562731E-47.9746493
TTCGAAA4350.00168993847.91755256
TTCAATC11407.2759576E-127.8532562
GTTCAAT11203.092282E-117.73706441
GATTCCG4550.00254595287.59420552
GTTGACG4100.0087850627.5602976
GTCGAAG4150.0092463377.51707121
GTTGAAT16850.07.1998321
TCACCCG7755.3936947E-67.1093653
TCTCGAT5400.00175338067.02813548
CGGGAAT6057.307752E-46.87511731
GGGAAAT25850.06.8385471
GGGTAAT6108.0258073E-46.81876371
GGCTAAT8005.8284553E-56.4991341