FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4171235
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1759.094947E-1225.6267231
CGACCGT2352.0008883E-1120.55170632
GTTCGAC2802.564775E-1017.24875329
TTCGACC3106.002665E-1116.69234330
TCGCGTA1756.375443E-515.76783166
GTCGGTT4201.8189894E-1214.9747911
TCGACGC3252.1245796E-914.85779243
GTCGGAA5750.014.5841441
CGCGTAA1901.2854011E-414.52300367
AGTTCGA3506.028131E-913.79701728
GTTCCGT4801.4551915E-1113.10294151
AAGTTCG3952.3337634E-913.09859227
CGATCGG2406.38991E-512.93423841
GCGATCG2601.3500701E-411.93929840
TCTCGAC4207.6899596E-811.49710141
GTCCGTT5259.404175E-1011.3142871
CCCGTAT3155.7531048E-511.0924381
GTCGCGT2200.006325150410.97476965
GTCGGAT5158.385541E-910.8555131
CCGGTAT3551.623172E-510.8268431