FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14389416
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTC19800.08.54070850
GATTCCG18350.08.09761252
CTCGATT18350.08.08666849
ATTCCGT20350.07.811204453
TTCTCGA20750.07.318392347
TCTCGAT18650.07.216440748
CGATTCC25900.06.668222451
GTTTGAT83700.06.6247441
GTTGAAT59950.06.4744581
TACATCG28300.06.44864753
ACTAGGC18750.06.426530411
GTTTATT93250.06.39225671
CGCGGTA7056.3753844E-46.349698517
GTTAAAT66200.06.28199861
CCGCTAG8309.9874655E-56.25078965
GTTTTTT190500.06.18526841
TCACCCG25500.06.07647283
GGAAAAT134750.05.9924131
AATCGAA23700.05.95705135
CGCTAGA7550.00137966445.95553966