FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14389416
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG5400.019.80374131
GTTCGAC8200.014.30356929
GTCCGTT17450.013.8070111
CGACCGT7800.013.71028132
GTCGGGT21000.012.1380681
GTCCGCT17350.012.07529451
TCGACGC10050.012.01198843
GTCGTTT25900.011.8639391
GTCGGTT11900.011.4436251
GTCGCGA3155.7948673E-511.0849931
GCCGTAT15150.010.6020831
CCCGTAT10550.010.2601851
TTCGACC11100.010.25581730
GTCCAGT68500.010.1439831
GTCCGAT17300.010.0918281
AGTTCGA12350.010.0545728
GTCGAGA10850.09.9764941
GTCCGAA14350.09.9764941
TCGCGTA5704.9620212E-89.68207866
GTCGGCT21700.09.6546721