FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6731848
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT3451.2952703E-511.0499271
TCGGTCG2650.002074995310.43599454
CGATACG7403.8580765E-98.8408129
TTGCGTA5251.670287E-58.52566716
CGGATCG4109.4330235E-48.3987623
CTCGATT7202.0045081E-78.14674849
ACTATCG6301.8663244E-57.678157352
CAATCGG10505.9117156E-107.54400974
GTTTAAT34050.07.2264841
CTTCAAT32300.07.18882751
CTATCGG5855.1643065E-47.0875353
ACGGAAT10302.3546818E-87.038175633
TACGATA8402.3914545E-66.9688727
GTTTATT42600.06.9150461
GATACGG11051.3080353E-86.85531430
TCGTCGG6057.7006436E-46.843743348
GTCGGCT8201.1340355E-56.76226041
GCGAATC5100.0079958786.7517962
CAATCCG6250.0011379066.61248874
TACGGAA11008.363895E-86.585534632