FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6731848
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT7300.016.7350831
CGCTAGT2101.6252252E-514.9591131
GATCGCG2506.1321116E-613.79704640
GTCGAGA5350.013.04844951
GTCCGCT9100.012.2741461
GTCGGTT5451.0913936E-1112.1685761
GTCGTAT8100.012.0657881
CGCGAGT2902.823535E-511.89382943
GTCCGAA6650.011.5473871
TCGCGAG2752.2742574E-411.28840842
GTCGCAT5351.3078534E-911.0911821
TCGACCG2500.001299737111.03853931
CGATCGG4401.4637772E-710.97484241
GCCTTAT13300.010.7600651
GTCCAGT32500.010.7398781
GTCGTTT12250.010.5426141
GCCGTAT7051.0913936E-1110.3971151
GTCGGCT9450.010.3421051
ATCGCGA3351.225349E-410.29622741
GTCCGAT8150.010.2786551