FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753295
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT4408.58563E-1012.55055949
TCTCGAT5805.9608283E-910.11618348
TCGATTC5856.855771E-910.0303950
CGGTACA6302.0936568E-99.88994861
GATTCCG5301.738481E-79.77974852
GCGGTAC7252.401066E-109.54814760
GGCGGTA6604.6748028E-99.43951959
GTTAGAC5301.7531074E-69.1548181
GTTCGTT4201.1247855E-49.0769711
ATTCCGT6951.166336E-88.94952553
GTCTAAG7454.1600288E-98.80329544
AATCGTC3956.5132603E-48.7174735
CGATTCC6951.11316695E-78.45006851
TTCTCGA6151.5146397E-68.41929447
TCACCCG7851.1097654E-88.3336823
ACACACG10002.1827873E-118.29322652
CGGAAAT6009.337999E-68.0867561
GAATCGT4754.563908E-47.97440624
CCCGGAC7453.5784797E-77.856589311
TAGCATA9658.5310603E-107.85024269