FastQCFastQC Report
Tue 22 Nov 2016
WTCHG_328669_279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_328669_279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753295
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT2651.9354047E-917.136861
GTCGCTT5000.015.3704471
TCCGATC2952.3445791E-612.879652
ATCGTCG2952.3827488E-612.86111836
GTCCGCT4458.4764906E-1012.560121
GTCGAGT3103.6010515E-612.3955211
CCGCTAT2000.002823787512.2264911
GTCGGCT5451.0913936E-1112.1784181
CCGAATT3501.2209421E-611.8425872
AGTATCG3257.030778E-611.6742533
GTCGCGG4551.5792466E-811.5163181
CGATCGG2409.244259E-411.49721341
GGCGAAT3358.552528E-611.4704821
CTCGTAT5208.058123E-1011.4203481
GTCGTGA4004.3066757E-711.353171
GTCCGAT4105.925631E-711.0762641
GCGATCG2500.001300698511.03732540
CGCAAAT2852.814927E-411.0314211
GCCCGTT7350.010.9313631
CGAATCT3803.304829E-610.9013913